Doxycycline Rodent Diets

Teklad diets provides a range of research diet options, including diet containing doxycycline for your Tet-regulated systems. Commonly requested Teklad Diet TD.01306 containing 625mg/kg of doxycycline hyclate added to our base rodent chow 2018 is stocked and available for shipping within 2 days for non-irradiated diet or 2 weeks for irradiated diet. Highly customizable, our nutritionists will work with you to tailor the base diet, doxycycline concentration, and food coloring to meet your specific requirements. If the options below do not meet your needs, contact a nutritionist to discuss a custom solution.

Browse our curated list of doxycycline references for additional information.

Watch our Teklad Tek Talk on Doxycycline Diets featuring Dr. Barb Mickelson.

Formula examples with Teklad global 2018 base diet

Doxycycline hyclate¹ (mg/kg diet)	Natural color	Alternatives with food coloring
46	TD.10483	TD.180327 Red TD.120240 Blue
100	TD.04233	TD.130840 Blue
200	TD.00502	TD.180625 Red TD.150431 Blue TD.04104 Green
625	TD.01306 stocked²	TD.08541 Red TD.120769 Blue TD.09628 Green
1000	TD.05298	TD.06294 Red TD.120658 Blue
2000	TD.05512	TD.140011 Red TD.09633 Green
6000	TD.01533

¹ Doxycycline hyclate is ~87% doxycycline
² TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

Considerations when choosing a base diet

Teklad Global Rodent Diets^® like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For different doxycycline concentrations or base diets, contact our expert nutritionists.

Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate¹ at 625 mg/kg diet

Base diet	Natural color	Alternatives with food coloring
2014	TD.00426	TD.07382 Red TD.07573 Yellow
2016	TD.02503	TD.140263 Blue TD.170364v Red
2019	TD.09651	TD.09761 Green
2020	TD.110720	TD.130141 Red

¹ Doxycycline hyclate is ~87% doxycycline

Advantages of diet delivery of doxycycline

Typically the same doxycycline concentration can be utilized in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
Diet containing doxycycline is very stable for at least 6 months (Redelsperger, et. al. 2016), whereas using a water vehicle will require weekly changes.
Administering the compound through diet instead of water could also decrease the risk of dehydration and eliminate the need to add sucrose (Cawthorne et al. 2007).

Key planning information

Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month.
Cold storage (refrigerator or freezer) is the best practice for doxycycline-containing diet that is not irradiated. Irradiated diet can be stored cool and dry.
While the default recommendation for shelf life is six months, doxycycline is stable longer (Redesperger, et. al. 2016). We recommend that use up to nine months is reasonable based on overall doxycycline stability in the diet along with nutritional adequacy of the base diet. Note that shelf life policies of a given institution may take precedence over this assessment.
Lead time for shipment is approximately 2 weeks for non-irradiated and 4 weeks for irradiated diet.
TD.01306 ships within 2 days for non-irradiated or 2 weeks for irradiated diet.
Irradiation (20 - 50 kGy) is optional, and must be requested at time of order.

Safe handling of doxycycline diets

Doxycycline is a tetracycline class antibiotic.
A usual dose for therapeutic effects in humans is 100-200 mg/day.
Doxycycline diets for rodents typically contain ~2 mg/pellet.
Accidental doxycycline exposure can be minimized by using typical lab precautions such as lab coat, gloves, and mask when handling the diet.
Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.

Browse our curated list of doxycycline references for additional information.

Contact our expert nutritionists to discuss your specific needs or custom formulation.