From Specialty Histology to Image Analysis, Our Experts Focus on the Insights Your Program Needs

Our specialized histology/pathology methods provide you with endpoint determinations that reflect tissue compartment localization and cell phenotype context. The outcome can then be further assessed through quantitative image analysis providing the data you need to make informed decisions. We are constantly developing new approaches to meet our clients' needs. If you do not see a capability you need listed below, please contact us.

• GLP compliance
• Robust QC and QA staffing
• Routine tissue trimming, paraffin embedding, and sectioning of rodent and non-rodent tissues
• Standard and oversized slides
• H&E staining
• Staining for brightfield and fluorescence microscopy
• Wide selection of special stains
• Serial and step sectioning
• OCT embedding and cryosectioning for H&E, special, and Immunohistochemistry (IHC) staining
• Customized bone decalcification methods
• Extensive experience in:
  • Customized trimming and sectioning of bone, ocular, and OTICSpecific and complex brain tissue trimming and processing
  • Medical device support

From routine applications to more complicated procedures, Inotiv has the expertise and the capability to determine the ideal specialty stain for your research needs. Our talented histotechnicians continuously create the highest quality novel stains that highlight what you need to move your program forward.

At Inotiv, you’ll benefit from industry-recognized expertise in Image Analysis (IA). Consultation and design-based techniques, coupled with broad histochemical/immunohistochemical capabilities and knowledge of disease, ensure we deliver the best and most efficient quantitative evaluations possible to accelerate your development project.

We offer:

• Computer assisted, high-throughput capabilities
• Conducted on brightfield or fluorescent whole slide scanned images 
• Artificial Intelligence (AI) applied across multiple platforms, including Visiopharm^® or inForm^® 
• Visiopharm^® image analysis platform allows for analysis of H&E, special stain, IHC, and ISH, including multiplex analysis and endogenous fluorescent analysis
• High-quality quantitative data for applications, including but not limited to:
  
  • Muscle fiber cross-sectional area and Feret’s diameter
  • Co-expression and cell classification
  • Biodistribution of proteins or nucleic acids of interest
  • Disease morphology (e.g., fibrosis, lipid, necrosis, apoptosis)
  • Immunologic and oncologic endpoints
  • Object count and size quantitation (e.g., vessels, amyloid plaques, glomeruli, islets)
  • Glial cell assessment
  • Proliferation assays
  • Spatial analysis
• Pathologist oversight of all analyses, including prepared illustrative images and interpretive reports

Our team creates high-resolution whole slide images, which can then be seamlessly analyzed by quantitative image analysis or pathology evaluation.

Our whole slide imaging (WSI) capabilities include:

• WSI using the Leica Aperio AT2, Akoya PhenoImager HT 2.0, or Slideview VS200
• Standard or large format slide sizes for H&E, IHC, IF, ISH, and special stains
• Resolutions of 500 nm/pixel at 20x magnification or 250 nm/pixel at 40x magnification
• Online image hosting for easy review of study
• Zoom in and out, pan around the whole slide image just like a microscope using our web-based image management system, Proscia-Concentriq
  • Simple, secure online access from any web browser, on any computer, tablet, or mobile device
• Digital data transfer
  • Whole slide images can be delivered by internet transfer to your organization’s AWS bucket or other cloud storage systems
• Images are compatible with most third-party image-analysis software

At Inotiv, we are committed to providing you with exceptional immunohistochemistry (IHC) and immunofluorescence lab services. Our extensive expertise with advanced techniques across a wide array of disease models allows us to produce IHC and IF staining that research and discovery organizations rely on for accurate assessments of their preclinical programs. Our ability to provide specialized approaches to your complex needs, while still maintaining quick turnaround times and high quality, makes Inotiv your partner of choice with the ideal source for reliable IHC and IF lab services.

Here’s how we can support you:

• Method development
• New antibody protocol optimization
• Custom antibody panels
• Immunophenotyping markers
• Variety of cell type specific markers across multiple species
• Transduced proteins and tags
• Bone and other specialty tissues
• Paraffin and frozen sections
• Staining for Bright field and fluorescence microscopy
• Antibody validation
• Project specific R&D
• Automated quantification of IHC including Nuclear, Membrane, Cytoplasm, and CD markers

Inotiv can help with other aspects of your project, such as method or protocol development and rushed completion requirements.

We offer the ISH technique to bring you faster and more accurate results when quantifying your nucleic acid targets across tissues and cell lines.

This includes:

• Custom-made probes to identify specific gene expression and miRNA, lncRNA, and viral DNA genomes
• Frequently used as an adjunct to assess IHC validity
• Multiplex staining
  • ISH combined with IHC capabilities
  • Multiplex FISH

Below you can access our IHC antibody list. We can accommodate other specific or modified stains and/or procedures not listed. We are adding new antibodies weekly, so please contact us to request an antibody not listed.

• Evaluation of microscopic slides; our pathologists are available to travel or slides may be shipped to us
• Full narrative pathology reports
• Pathology data, to include organ and body weight correlations
• Clinical pathology correlations to histopathology and interpretations
• Pathology Working Groups
• Consulting